期刊
TREE PHYSIOLOGY
卷 41, 期 7, 页码 1278-1288出版社
OXFORD UNIV PRESS
DOI: 10.1093/treephys/tpaa179
关键词
ethylene signaling; gene expression; rubber biosynthesis; sucrose transporter; transcription factor
类别
资金
- National Natural Science Foundation of China [31825007]
- Starting Research Fund of Hainan University
- French Agricultural Research Centre for International Development
The study identified five Ethylene Response Factor (ERF) genes in the cDNA library of Hevea latex, with HbERF-IXc4 showing the strongest transactivation capacity and being most frequently screened. These ERFs mainly localized in the nucleus and four of them exhibited apparent transactivation activity. This indicates the critical involvement of ERFs, especially HbERF-IXc4, in the activation of HbSUT3 expression in latex after Ethrel treatment and the stimulated latex yield.
Natural rubber is an important industrial raw material and is commercially produced by rubber trees (Hevea brasiliensis). The sucrose transporter HbSUT3 plays an essential role in rubber production. Its expression in latex (cytoplasm of rubber-producing laticifers) is induced by bark treatment with Ethrel, an ethylene releaser, and the inducing effect correlates well with Ethrel-stimulated rubber yield increase. However, the mechanisms of ethylene induction on HbSUT3 expression are not known. Here, five Ethylene Response Factor (ERF) genes were identified from the cDNA library of Hevea latex by yeast one-hybrid screening with the promoter of HbSUT3 gene as bait. As revealed in a tobacco (Nicotiana tabacum) protoplast transient expression system, these HbERFs were mainly localized in the nucleus and four of them exhibited apparent transactivation activity. Of the five HbERF genes, HbERF-IXc4 was the most frequently screened in yeast one-hybrid, accounting for 65% of the ERF clones obtained. Moreover, among the five HbERFs, HbERF-IXc4 showed the strongest transactivation capacity when expressed in tobacco protoplast, the highest transcript abundance in latex and a close expressional correlation with its target gene, HbSUT3, in response to the Ethrel treatment. Taken together, our results indicate that ERFs, especially HbERF-IXc4, are critically involved in the activation of HbSUT3 expression in latex after Ethrel treatment on Hevea bark, and thus the stimulated latex yield.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据