Preparation of Targeted Nano-Microbubble Contrast Agent and Its Application in the Diagnosis of Prostate Cancer

The objective of this study was to explore the application value of microbubble contrast materials in the diagnosis of prostate cancer. Firstly, ordinary nano-microbubbles and biotinylated nano-microbubbles were prepared by rotary evaporation method and mechanical vibration method. Then, the biotinylated anti-six-transmembrane prostate epithelial antigen-1 (STQWK-1) antibody was connected with the previously prepared nano-microbubbles with the help of biotin-avidin method, so as to generate a nano-microbubble contrast agent targeting prostate PC3 cells. While the contrast agent was characterized based on the immunofluorescence method, a microscope was applied to observe the in vitro targeting performance of targeted nano-microbubbles on PC3 cells. A prostate PC3 cell nude mouse transplanted tumor model was established, so that the contrast -enhanced ultrasound (CEUS) effect of different cotrast agents on transplanted tumor was compared, which IP: 49.249.253.194 On: Mon, 25 Oct 2021 06:11:30 were applied in CEUS examination of prostate cancer patients. The results showed that the targeted nano -Copyright: American Scientific Publishers microbubbles could display circular green fluorescence under the microscope, and the blank nano-microbubbles Delivered by Ingenta had no display under the microscope. Both ultrasonic microbubbles were circular and evenly distributed. The in vitro targeting experiments indicated that targeted nano-bubbles could accumulate on the surface of prostate cancer PC3 cells, and tumor transplantation model proved that the targeted contrast agent carrying STQWK-1 antibody had a strong development and enhancement effect. In the diagnosis of prostate cancer patients, the sensitivity (83.3% vs. 52%), specificity (66.7% vs. 36.3%), accuracy (75% vs. 47.2%), and other indicators of the diagnosis of targeted nano-bubbles prepared showed marked advantages compared with routine trans-rectal US (TRUS) diagnosis. The contrast agent prepared in this study could be specifically targeted to prostate PC3 cells in vitro, which had the effect of development and enhancement and had a good diagnostic performance in the diagnosis of prostate cancer.

Automated summary

This study focused on exploring the application value of microbubble contrast agents in the diagnosis of prostate cancer. By preparing targeted nano-microbubbles, the study demonstrated a strong targeting performance and enhancement effect in vitro and in vivo experiments. Compared with routine trans-rectal ultrasound (TRUS) diagnosis, the targeted nano-bubbles showed marked advantages in terms of sensitivity, specificity, and accuracy in diagnosing prostate cancer.