期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 32, 期 1, 页码 740-753出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2021.1991281
关键词
Acetamiprid; immunoassay; inner filter effect; gold nanoparticles; upconversion nanoparticles
资金
- National Key Research and Development Program of China [2017YFF0210200]
- National Natural Science Foundation of China [31772231]
The study introduces an immunoassay method for detecting acetamiprid, with optimized sensitivity and accuracy. The assay shows no cross-reactivity with analogues of acetamiprid, and correlates well with high-performance liquid chromatography for blind sample detection.
In this study, we present an inner filter effect-based immunoassay (IFE-IA) for the detection of acetamiprid with a turn off-on model. In the IFE-IA, the monoclonal antibody (mAb) conjugated with upconversion nanoparticles (UCNPs) were set as a fluorescence source, and the antigen conjugated with gold nanoparticles (AuNPs) were set as the fluorescence switch off reagent, while acetamiprid acts as the fluorescence turn-on reagent. After optimization, the concentration of acetamiprid producing 50% saturation of the signal (SC50) was 0.04 mu g/L, and the detection range (SC10-SC90) was 0.002-0.58 mu g/L. The IFE-IA showed no cross-reactivity with the analogues of acetamiprid except for thiacloprid (36.4%). The average recoveries of the immunoassay for spiked samples were in the range of 75.1-104.7%. In addition, the assay showed good correlation with high-performance liquid chromatography in the detection of blind samples.
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