期刊
EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY
卷 42, 期 5, 页码 957-965出版社
IMR PRESS
DOI: 10.31083/j.ejgo4205144
关键词
Icariin; Breast cancer; MELK; PI3K/AKT signaling pathway
资金
- Nanjing Science and Technology Project [201803052, CLB20J023]
The study revealed that icariin promotes apoptosis of breast cancer cells by regulating the PI3K/AKT signaling pathway mediated by MELK. Icariin inhibits cell proliferation, induces G2/M arrest, and reduces migration ability of breast cancer cells. Overall, icariin shows therapeutic potential for breast cancer by inhibiting MELK expression and PI3K/AKT pathway.
Objective: To investigate the mechanism of icariin in promoting apoptosis of breast cancer cells by regulating the Phosphatidylinositol kinase (PI3K) PI3K/AKT signaling pathway mediated by Maternal embryonic leucine zipper kinase (MELK). Methods: Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting were used to detect the expression of MELK. small interfering RNA (siRNA)-M ELK cell transfection technology was used to detect the correlation between MELK and PI3K/AKT. Different icariin concentrations on proliferation, migration and apoptosis of breast cancer cells were detected by flow cytometry, CCK-8, and Transwell. Western blot was used to detect the effects of icariin on MELK expression, AKT, cyclin, and apoptosis-related proteins. Results: Compared with normal mammary epithelial cells, the expression of MELK in breast cancer cells was significantly increased (p < 0.01). si-MELK decreased the expression of p-AKT, increased the expression of epithelial-mesenchymal transition (EMT)-related protein E-cadherin, and decreased the expression of N-cadherin and vimentin. Compared with the control group, icariin solution group showed a decrease in cell proliferation ability, a significant increase in cell apoptosis and a decrease in cell migration ability (p < 0.05). Icariin could induce G2/M arrest and inhibit the growth of breast cancer cells. Conclusion: Icariin can inhibit the expression of MELK, inhibit the PI3k/AKT signaling pathway to a certain extent, and further has a therapeutic effect on breast cancer.
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