期刊
CHEMICAL COMMUNICATIONS
卷 57, 期 90, 页码 11960-11963出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc04916j
关键词
-
资金
- European Union [814400]
This method involves immobilising and stabilising enzymes on the surface of silica nanoparticles through reversible covalent binding, resulting in high and durable activity retention and enzyme stabilisation.
We report a method of glycosylated enzymes' surface immobilisation and stabilisation. The enzyme is immobilised at the surface of silica nanoparticles through the reversible covalent binding of vicinal diols of the enzyme glycans with a surface-attached boronate derivative. A soft organosilica layer of controlled thickness is grown at the silica surface, entrapping the enzyme and thus avoiding enzyme leaching. We demonstrate that this approach results not only in high and durable activity retention but also enzyme stabilisation.
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