期刊
ANALYST
卷 146, 期 22, 页码 6831-6839出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1an01748a
关键词
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资金
- National Key RD Program [2019YFC1604704]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_1841]
A highly specific and sensitive monoclonal antibody against quinclorac was prepared in this study, and an ic-ELISA and an immunochromatographic strip assay were established for Qui detection. The analysis results were consistent with LC-MS/MS, indicating that the established methods can provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.
In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.763 ng mL(-1). No cross-reaction with other quinolines indicated that mAb 2G3 had high specificity. The recovery of the established ic-ELISA method was in the range of 85.6% to 98.9%. The cut-off value of Qui in cucumber and tomato by immunochromatographic strip was 200 ng g(-1). The analysis results of ic-ELISA and immunochromatographic strip assay were consistent with the results of LC-MS/MS, which further proved that the established ic-ELISA and immunochromatographic strip assay could provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.
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