期刊
PLANT PHYSIOLOGY
卷 187, 期 2, 页码 590-602出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiab278
关键词
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资金
- Gatsby Charitable Foundation
- ERC under the European Union [759282]
- European Research Council (ERC) [759282] Funding Source: European Research Council (ERC)
Phytohormones play a critical role in regulating plant growth, with their levels and distribution being highly dynamic over space and time. Fluorescent biosensors are ideal tools for tracking these dynamics with high spatiotemporal resolution.
Phytohormones act as key regulators of plant growth that coordinate developmental and physiological processes across cells, tissues and organs. As such, their levels and distribution are highly dynamic owing to changes in their biosynthesis, transport, modification and degradation that occur over space and time. Fluorescent biosensors represent ideal tools to track these dynamics with high spatiotemporal resolution in a minimally invasive manner. Substantial progress has been made in generating a diverse set of hormone sensors with recent FRET biosensors for visualising hormone concentrations complementing information provided by transcriptional, translational and degron-based reporters. In this review, we provide an update on fluorescent biosensor designs, examine the key properties that constitute an ideal hormone biosensor, discuss the use of these sensors in conjunction with in vivo hormone perturbations and highlight the latest discoveries made using these tools.
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