期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 17, 期 15, 页码 4340-4352出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.64675
关键词
Cepharanthine; Hepatocellular carcinoma; Metabolism; Apoptosis
资金
- National Natural Science Foundation of China [11832008, 11532004, 11902058]
- Innovation and Attracting Talents Program for College and University (111 Project) [B06023]
- Ministry of Education [CQKLBST-2017-011]
Cepharanthine (CEP), extracted from Stephania cepharantha Hayata, has shown potential in treating hepatocellular carcinoma (HCC) by inhibiting cell migration, proliferation, and inducing apoptosis. Through RNA-seq and GC-MS, CEP was found to affect metabolism-associated pathways and down-regulate metabolites, leading to significant antitumor effects in both in vitro and in vivo experiments.
Cepharanthine (CEP), a natural compound extracted from Stephania cepharantha Hayata, has been found to have the potential to treat a variety of tumors in recent years. This study aims to evaluate the anti-hepatocellular carcinoma (HCC) effect of CEP and determine its in-depth mechanism. In this study, Hep3B and HCCLM3 cells were selected to evaluate the antitumor effects of CEP in vitro, whereas tumor xenograft in nude mice was performed to make in vivo anti-tumor assessment. RNA-sequence (RNA-seq) was used to identify possible molecular targets and pathways. Further, gas chromatography mass spectrometry (GC-MS) was performed to assess the differential metabolites involved in mediating the effect of CEP on the HCC cell line. Our results showed that CEP treatment resulted in the dose-dependent inhibition of cell viability, migration, and proliferation and could also induce apoptosis in HCC cells. RNA-seq following CEP treatment identified 168 differentially expressed genes (DEGs), which were highly enriched in metabolism-associated pathways. In addition, CEP down-regulated many metabolites through the amino acid metabolism pathway. In vivo experiment showed that CEP significantly suppressed tumor growth. Our results indicate that CEP has significant antitumor effects and has the potential to be a candidate drug for HCC treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据