期刊
ANALYST
卷 146, 期 23, 页码 7207-7217出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1an01458g
关键词
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资金
- NSERC Alliance COVID-19 grant
- Trent U
- Trent U Graduate Studies and Environmental and Life Sciences Program for the International Graduate Scholarship
This study developed a novel method for precise detection of the S1 spike protein of SARS-CoV-2, showing high selectivity and specificity with excellent performance in terms of dynamic range and limit of detection. Utilizing aptamer technology, the method demonstrated good stability and repeatability in real-time monitoring.
In this work, we designed and developed a method to detect S1 spike protein of SARS-CoV-2. The portable Localized Surface Plasmon Resonance instrument equipped with a two-channel system was combined with the biotin-streptavidin platform on a nanogold surface to immobilize biotinylated aptamers. The proposed assay does not utilize antibodies or enzyme-based reagents, further simplifying the detection method. Using aptamer-protein bioaffinity interactions, the aptasensor selectively and specifically detected in real-time S1 spike protein, rather than S2 spike protein, RBD spike protein, or bovine serum albumin. The dynamic range and limit of detection of the aptasensor was determined to be 1 nM-100 nM and 0.26 nM, respectively. Notably, aptasensor detected preferentially S1 protein of SARS-CoV-2 compared to SARS-CoV and detected S1 protein with >95% recovery in artificial saliva, and serum albumin, excellent repeatability and shelf-life stability. The method may provide a low-cost, rapid, and real-time detection and monitoring of viruses in the general public.
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