期刊
NATURE PLANTS
卷 7, 期 11, 页码 1461-1474出版社
NATURE PORTFOLIO
DOI: 10.1038/s41477-021-01008-7
关键词
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资金
- Ohio State Cellular, Molecular and Biochemical Sciences Training Program [NIH T32 GM086252]
- National Science Foundation Federation [MCB-1908521]
The study found that siRNA-guided ARGONAUTE first recruits polymerase V to new target sites without pre-existing DNA methylation and triggers the cycle of RdDM at the target sites, thereby establishing epigenetic silencing.
In mammals and plants, cytosine DNA methylation is essential for the epigenetic repression of transposable elements and foreign DNA. In plants, DNA methylation is guided by small interfering RNAs (siRNAs) in a self-reinforcing cycle termed RNA-directed DNA methylation (RdDM). RdDM requires the specialized RNA polymerase V (Pol V), and the key unanswered question is how Pol V is first recruited to new target sites without pre-existing DNA methylation. We find that Pol V follows and is dependent on the recruitment of an AGO4-clade ARGONAUTE protein, and any siRNA can guide the ARGONAUTE protein to the new target locus independent of pre-existing DNA methylation. These findings reject long-standing models of RdDM initiation and instead demonstrate that siRNA-guided ARGONAUTE targeting is necessary, sufficient and first to target Pol V recruitment and trigger the cycle of RdDM at a transcribed target locus, thereby establishing epigenetic silencing. This study finds that siRNA-guided ARGONAUTE first recruits polymerase V to new target sites without pre-existing DNA methylation and triggers the cycle of RdDM at the target sites, thereby establishing epigenetic silencing.
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