期刊
GREEN CHEMISTRY
卷 23, 期 23, 页码 9447-9453出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1gc03056f
关键词
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资金
- Basic Science Research program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT, and Future Planning [2020R1A2C2009806]
- National Research Foundation of Korea [2020R1A2C2009806] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A multienzyme biocatalytic cascade was developed to synthesize nylon monomers with high efficiency and a self-sufficient redox network, utilizing a cell-based modularization strategy to produce 6-aminohexanoic acid and epsilon-caprolactam from cyclohexanol. The designed E. coli consortia showed high yields of the desired products, making it a cost-effective method for nylon precursor production.
We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and epsilon-caprolactam from cyclohexanol. The designed consortia of E. coli containing the respective modules was found to produce >97% of 6-aminohexanoic acid (from 100 mM cyclohexanol) and 28% of caprolactam (from 10 mM cyclohexanol) with 2-fold excess of propylamine in a one-pot reaction. The use of cheaper substrates and co-substrates makes it an economically beneficial approach for the production of nylon precursors.
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