期刊
RSC ADVANCES
卷 11, 期 57, 页码 36360-36366出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ra04063d
关键词
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资金
- National Key R&D Program of China [2018YFA0703100, 2018YFC1105402, 2017YFC1104402, 2020YFB0204803]
- National Natural Science Foundation of China [31900959, 31700823, U1801252, 31771027]
- Natural Science Foundation of Guangdong Province of China [2020A1515011354]
- Fundamental Research Funds for the Central Universities [2019MS003]
- Guangdong Natural Science Funds for Distinguished Young Scholars [2019B151502029]
- Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory [2018GZR110102001]
- Key-Area Research and Development Program of Guangdong Province [2019B010940001]
- Funds for Young Pearl River Scholars
The study investigated the behavior of osteopontin (OPN) on different self-assembled monolayers (SAMs) with specific chemical groups, finding that SAMs-NH2 strongly adsorbed OPN while SAMs-OEG had the lowest amount of OPN. In vitro cell assay results showed that pre-adsorbed OPN on SAMs promoted the behavior of mouse bone marrow mesenchymal stem cells.
Osteopontin (OPN) is an important protein for mediating cell behaviour on biomaterials. However, the interactions between the chemical groups on the biomaterial surface and OPN still need to be further clarified, which has restricted the application of OPN in biomaterial functionalization. In the present study, we developed different self-assembled monolayers (SAMs) with specific chemical groups, including SAMs-OH, SAMs-OEG, SAMs-COOH, SAMs-NH2, and SAMs-PO3H2, to study the behavior of OPN on these SAMs. The results showed that SAMs-NH2 could strongly adsorb OPN, and the amount of protein was highest on this material. Meanwhile, the lowest amount of OPN was present on SAMs-OEG. Interestingly, the unit-mass trend of bound OPN monoclonal antibodies (mAbs) on the SAMs was opposite to the OPN adsorption trend: lowest on SAMs-NH2 but highest on SAMs-OEG. In vitro cell assay results showed that mouse bone marrow mesenchymal stem cells (mBMSCs) on SAMs-COOH, SAMs-NH2, and SAMs-PO3H2 with pre-adsorbed OPN showed promoted behaviour, in terms of spreading, viability, and the expression levels of alpha(v) and beta(3) genes, compared with the other two SAMs, demonstrating the higher bioactivity of the adsorbed OPN. We believe that our findings will have great potential for developing OPN-activated biomaterials.
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