期刊
GREEN PROCESSING AND SYNTHESIS
卷 10, 期 1, 页码 700-710出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/gps-2021-0056
关键词
TiO2 NPs; MALDI mass spectrometry; lipid
资金
- National Natural Science Foundation of China [11475020, 11975048]
- Beijing Union University Graduate Program
The study explored a methodology using TiO2 NPs as a matrix for lipid determination with MALDI MS. It found that TiO2 NPs are preferable for determining lipid standards and mixed lipids, and could be a potential direction for future research on matrix development.
The structures of lipids are diverse, and thus, lipids show various biological functions. Systematic determination of lipids in organisms has always been a concern. In this paper, a methodology on the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), with titanium dioxide nanoparticles (TiO2 NPs) as the matrix, was studied for lipid determination. The results showed that the following conditions were preferable in the determination of small-molecule lipids (such as hypoxanthine, guanosine, uridine, and cytidine), lipid standards (such as GC, GM, TG, phosphatidylethanolamine, phosphatidylcholine, and ceramide), and mixed lipids (extracted from brain homogenate with methanol alone and with the B&D method): TiO2 NPs as the matrix, absolute ethanol as the solvent, 1 mg of TiO2 NPs dispersed in 1 mL of absolute ethanol as the matrix solution, NaCl as the ionization reagent, and positive mass spectrometry (MS) as the mode. Modified TiO2 NP as a new matrix for MALDI MS will be a future research direction; in addition, the characteristics of TiO2 NPs make it a potential matrix for imaging MS.
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