期刊
PLANT COMMUNICATIONS
卷 2, 期 6, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xplc.2021.100232
关键词
transcription factor; bHLH; target specificity; plants
资金
- Spanish Ministry of Science and Innovation [BIO2017-86651-P, BIO2016-77216-R]
Recent research has partially decoded the cis-regulatory code in eukaryotes, showing that A/T-rich modules flanking the MYC-binding motif are essential for TF recognition in plants. Different bHLHs may have their own codes for DNA binding and specific target selection, with TF-DNA interplay playing a crucial role, especially in the case of MYCs.
Transcription factors (TFs) regulate gene expression by binding to cis-regulatory sequences in the promoters of target genes. Recent research is helping to decipher in part the cis-regulatory code in eukaryotes, including plants, but it is not yet fully understood how paralogous TFs select their targets. Here we addressed this question by studying several proteins of the basic helix-loop-helix (bHLH) family of plant TFs, all of which recognize the same DNA motif. We focused on the MYC-related group of bHLHs, that redundantly regulate the jasmonate (JA) signaling pathway, and we observed a high correspondence between DNA-binding profiles in vitro and MYC function in vivo. We demonstrated that A/T-rich modules flanking the MYC-binding motif, conserved from bryophytes to higher plants, are essential for TF recognition. We observed particular DNA-shape features associated with A/T modules, indicating that the DNA shape may contribute to MYC DNA binding. We extended this analysis to 20 additional bHLHs and observed correspondence between in vitro binding and protein function, but it could not be attributed to A/T modules as in MYCs. We conclude that different bHLHs may have their own codes for DNA binding and specific selection of targets that, at least in the case of MYCs, depend on the TF-DNA interplay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据