期刊
PAKISTAN JOURNAL OF ZOOLOGY
卷 53, 期 6, 页码 2417-2425出版社
ZOOLOGICAL SOC PAKISTAN
DOI: 10.17582/journal.pjz/20200109070125
关键词
Bovine mastitis; Staphylococcus aureus; RAPD; Genetic polymorphisms
类别
资金
- National Key Research and Development Program [2016YFD0501305]
This study conducted genotyping of Staphylococcus aureus isolates from clinical mastitis milk samples collected from dairy farms in 5 different provinces in China using the RAPD method. The results showed noticeable differences in genotype distribution among provinces, which may be attributed to variations in dairy farming practices, geographical environments, and antibiotic usage.
In order to provide science-based information for the control of cow mastitis, to understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shanghai, Sichuan, and Gansu. The results showed that the amplified product bands with three primers per isolate ranged from 1 similar to 9 and the product sizes from 240 to 4,500 bp. Primer OLP13 had the highest discriminant coefficient. The Staphylococcus aureus strains were divided into 11 genotypes by fingerprint with oligonucleotide primers OLP13. There were 12 strains in type 1, 8 in each of types 2 and 5, 22 in each of types 3 and 7, 13 in types 4, 11 in type 6, 25 in type 8, 28 in type 9, 8 in type 10 and 5 in type 11. Among the 11 types of S. aureus isolates, type3 (14/37) and type 7 (15/35) were the main prevalent strains in Guizhou and Inner Mongolia Province respectively. Type 9 (21/55) had the highest prevalence rate and type 8 (10/55) was higher than other types in Shanghai, both of them were closely related. The results showed that the genotype distribution in various regions was noticeably different. Differences in dairy farming practices, geographical environments, and the use of antibiotics may be the reasons for the varied distribution of different genotypes in those provinces.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据