期刊
LAB ON A CHIP
卷 21, 期 24, 页码 4814-4822出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1lc00744k
关键词
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类别
资金
- Chan Zuckerberg Initiative [2018-191850]
- National Institutes of Health [R01NS110665, R61NS112445, R21NS116257, RF1MH123971]
- National Science Foundation [2033800, 1706155]
- Vanderbilt Interdisciplinary Training Program in Alzheimer's Disease [T32 AG058524]
- Training Program in Environmental Toxicology [T32 ES007028]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [2033800] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1706155] Funding Source: National Science Foundation
Depositing a thin layer of parylene on 3D printed models allows them to be used as master molds for elastomeric device fabrication, making the prints biocompatible. Uncoated 3D prints can release toxic materials causing cell death, while parylene-coated prints can maintain cell viability and healthy morphology. This technique is simple and applicable for traditional microdevice fabrication and rapid prototyping of new designs.
Fabrication of microfluidic devices by photolithography generally requires specialized training and access to a cleanroom. As an alternative, 3D printing enables cost-effective fabrication of microdevices with complex features that would be suitable for many biomedical applications. However, commonly used resins are cytotoxic and unsuitable for devices involving cells. Furthermore, 3D prints are generally refractory to elastomer polymerization such that they cannot be used as master molds for fabricating devices from polymers (e.g. polydimethylsiloxane, or PDMS). Different post-print treatment strategies, such as heat curing, ultraviolet light exposure, and coating with silanes, have been explored to overcome these obstacles, but none have proven universally effective. Here, we show that deposition of a thin layer of parylene, a polymer commonly used for medical device applications, renders 3D prints biocompatible and allows them to be used as master molds for elastomeric device fabrication. When placed in culture dishes containing human neurons, regardless of resin type, uncoated 3D prints leached toxic material to yield complete cell death within 48 hours, whereas cells exhibited uniform viability and healthy morphology out to 21 days if the prints were coated with parylene. Diverse PDMS devices of different shapes and sizes were easily cast from parylene-coated 3D printed molds without any visible defects. As a proof-of-concept, we rapid prototyped and tested different types of PDMS devices, including triple chamber perfusion chips, droplet generators, and microwells. Overall, we suggest that the simplicity and reproducibility of this technique will make it attractive for fabricating traditional microdevices and rapid prototyping new designs. In particular, by minimizing user intervention on the fabrication and post-print treatment steps, our strategy could help make microfluidics more accessible to the biomedical research community.
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