期刊
CHEMICAL SCIENCE
卷 12, 期 46, 页码 15339-15346出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1sc05370a
关键词
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资金
- National Natural Science Foundation of China [21874103, 22074112]
- Central Funds Guiding the Local Science and Technology Development of Shenzhen [2021Szvup101]
The article introduces a novel method for accurate intracellular imaging of metal ions by utilizing an epigenetic demethylation-regulating DNAzyme system, which repairs and activates DNAzyme to achieve accurate imaging of metal ions.
The accurate intracellular imaging of metal ions requires an exquisite site-specific activation of metal-ion sensors, for which the pervasive epigenetic regulation strategy can serve as an ideal alternative thanks to its orthogonal control feature and endogenous cell/tissue-specific expression pattern. Herein, a simple yet versatile demethylation strategy was proposed for on-site repairing-to-activating the metal-ion-targeting DNAzyme and for achieving the accurate site-specific imaging of metal ions in live cells. This endogenous epigenetic demethylation-regulating DNAzyme system was prepared by modifying the DNAzyme with an m(6)A methylation group that incapacitates the DNAzyme probe, thus eliminating possible off-site signal leakage, while the cell-specific demethylase-mediated removal of methylation modification could efficiently restore the initial catalytic DNAzyme for sensing metal ions, thus allowing a high-contrast bioimaging in live cells. This epigenetic repair-to-activate DNAzyme strategy may facilitate the robust visualization of disease-specific biomarkers for in-depth exploration of their biological functions.
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