A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.

Automated summary

In this study, researchers synthesized a small-sized chromophore, BTTA-2OH, with favorable solubility, high two-photon excitation efficiency, and good fluorescence photostability to label living cell membranes. This allowed for the visualization of dynamic movement of membrane-related vesicles using a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.