Fluorescence Detection of miRNA-21 Using Au/Pt Bimetallic Tubular Micromotors Driven by Chemical and Surface Acoustic Wave Forces

In this study, surface acoustic wave (SAW) systems are described for the removal of molecules that are unbound to micromotors, thereby lowering the detection limit of the cancer-related biomarker miRNA-21. For this purpose, in the first step, mass production of the Au/Pt bimetallic tubular micromotor was performed with a simple membrane template electrodeposition. The motions of catalytic Au/Pt micromotors in peroxide fuel media were analyzed under the SAW field effect. The changes in the micromotor speed were investigated depending on the type and concentration of surfactants in the presence and absence of SAW streaming. Our detection strategy was based on immobilization of probe dye-labeled single-stranded probe DNA (6-carboxyfluorescein dye-labeled-single-stranded DNA) to Au/Pt micromotors that recognize target miRNA-21. Before/after hybridization of miRNA-21 (for both w/o SAW and SAW streaming conditions), the changes in the speed of micromotors and their fluorescence intensities were studied. The response of fluorescence intensities was observed to be linearly varied with the increase of the miRNA-21 concentration from 0.5 to 5 nM under both w/o SAW and with SAW. The resulting fluorescence sensor showed a limit of detection of 0.19 nM, more than 2 folds lower compared to w/o SAW conditions. Thus, the sensor and behaviors of Au/Pt tubular micromotors were improved by acoustic removal systems.

Automated summary

This study focuses on utilizing surface acoustic wave (SAW) systems to enhance the detection limit of the cancer-related biomarker miRNA-21 by immobilizing probe dye-labeled single-stranded DNA on Au/Pt micromotors that recognize the target miRNA-21. The speed and fluorescence intensity changes of the micromotors in response to miRNA-21 concentration variation were studied under both with and without SAW conditions, showing a linear relationship. The resulting fluorescence sensor demonstrated a lower limit of detection of 0.19 nM, which was more than 2 folds lower compared to conditions without SAW, indicating the effectiveness of acoustic removal systems in improving the sensor performance.