期刊
RSC ADVANCES
卷 11, 期 60, 页码 37917-37922出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ra07375c
关键词
-
资金
- Natural Science Foundation of Hubei Province [2020CFB193]
- Wuhan Municipal Science and Technology Bureau (Applied Foundation Frontier Project) [2019020701011440]
- Foundation of Jianghan University [2021KJZX001]
- Youth Talent Support Program
- Foundation of Cultivation of Scienti.c Institutions of Jianghan University
This study demonstrated a highly sensitive DNA detection method by combining OECT and HCR, showing great potential in improving detection efficiency and selectivity.
Highly sensitive detection of DNA is of great importance for the detection of genetic damage and errors for the diagnosis of many diseases. Traditional highly sensitive organic electrochemical transistor (OECT)-based methods mainly rely on good conductivity materials, which may be limited by complex synthesis and modification steps. In this work, DNA biosensor based on OECT and hybridization chain reaction (HCR) signal amplification was demonstrated for the first time. Au nanoparticles were electrochemically deposited on the Au gate electrode to increase the surface area. Then, the HCR products, long negatively charged double-stranded DNA, were connected to the target by hybridization, which can increase the effective gate voltage offset of OECT. This sensor exhibited high sensitivity and even 0.1 pM target DNA could be directly detected with a significant voltage shift. In addition, it could discriminate target DNA from the mismatched DNA with good selectivity. This proposed method based on HCR in DNA detection exhibited an efficient amplification performance on OECT, which provided new opportunities for highly sensitive and selective detection of DNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据