4.3 Article

Antagonistic regulation controls clathrin-mediated endocytosis: AP2 adaptor facilitation vs restraint from clathrin light chains

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Summary: This study utilized platinum replica and cryoelectron microscopy and tomography to provide a structural framework of clathrin-mediated endocytosis and determined the shape and size parameters associated with this process. It was found that clathrin sites maintain a constant surface area during curvature and flat clathrin can spontaneously curve into coated pits without additional energy sources or recruited factors. The generation of curvature was attributed to loosely connected and pentagon-containing flat lattices that rapidly curve when a flattening force is released.

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