4.3 Article

Notch signaling mediates olfactory multiciliated cell specification

期刊

CELLS & DEVELOPMENT
卷 168, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.cdev.2021.203715

关键词

Olfactory development; Multiciliated cell; Notch signaling; Cell fate specification

资金

  1. National Institutes of Health [R01HD100023]
  2. NSF-Simons Center for Quantitative Biology at Northwestern University
  3. NSF-Simons MathBioSys Research Center (Simons Foundation/SFARI) [597491-RWC]
  4. National Science Foundation [1764421]

向作者/读者索取更多资源

This study in zebrafish identified the earliest time window of olfactory MCC differentiation, showing that OMCC cells derive from peridermal cells and are regulated by the Notch signaling pathway. The research also found regionally segregated Notch signaling regulates the number of OMCCs and the differentiation-associated genes during olfactory development.
Epithelial multiciliated cells (MCCs) use motile cilia to direct external fluid flow, the disruption of which is associated with human diseases in a broad array of organs such as those in the respiratory, reproductive, and renal systems. While many of the signaling pathways that regulate MCC formation in these organ systems have been identified, similar characterization of MCC differentiation in the developing olfactory system has been lacking. Here, using live cell tracking, targeted cell ablation, and temporally-specific inhibition of the Notch signaling pathway, we identify the earliest time window of zebrafish olfactory MCC (OMCC) differentiation and demonstrate these cells' derivation from peridermal cells. We also describe regionally segregated Notch signaling across time points of rapid OMCC differentiation and show that Notch signaling downregulation yields an increase in OMCCs, suggesting that OMCC fate is normally repressed in a region-specific manner during olfactory development. Finally, we describe Notch signaling's regulation of the differentiation/ciliogenesis-associated genes foxj1a and foxj1b. Taken together, these findings provide new insights into the origins and developmental programming of OMCCs in vivo.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据