4.5 Article

Periostin gene expression in neu-positive breast cancer cells is regulated by a FGFR signaling cross talk with TGFβ/PI3K/AKT pathways

期刊

BREAST CANCER RESEARCH
卷 23, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s13058-021-01487-8

关键词

Periostin; Gene regulation; Breast cancer; FGF; AKT

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资金

  1. Canadian Breast Cancer Foundation - Canadian Institutes of Health Research
  2. Cancer Research Society
  3. Canadian Cancer Society Research Institute
  4. Canadian Institutes of Health Research

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Breast cancer is a highly heterogeneous disease with multiple drivers and complex regulatory networks. Postn plays a crucial role in tumor development, with about 50% of breast tumors acquiring Postn expression in epithelial tumor cells. The regulation of Postn expression involves cross-talk between FGFR, TGF beta, and PI3K/AKT pathways.
Background Breast cancer is a highly heterogeneous disease with multiple drivers and complex regulatory networks. Periostin (Postn) is a matricellular protein involved in a plethora of cancer types and other diseases. Postn has been shown to be involved in various processes of tumor development, such as angiogenesis, invasion, cell survival and metastasis. The expression of Postn in breast cancer cells has been correlated with a more aggressive phenotype. Despite extensive research, it remains unclear how epithelial cancer cells regulate Postn expression. Methods Using murine tumor models and human TMAs, we have assessed the proportion of tumor samples that have acquired Postn expression in tumor cells. Using biochemical approaches and tumor cell lines derived from Neu+ murine primary tumors, we have identified major regulators of Postn gene expression in breast cancer cell lines. Results Here, we show that, while the stromal compartment typically always expresses Postn, about 50% of breast tumors acquire Postn expression in the epithelial tumor cells. Furthermore, using an in vitro model, we show a cross-regulation between FGFR, TGF beta and PI3K/AKT pathways to regulate Postn expression. In HER2-positive murine breast cancer cells, we found that basic FGF can repress Postn expression through a PKC-dependent pathway, while TGF beta can induce Postn expression in a SMAD-independent manner. Postn induction following the removal of the FGF-suppressive signal is dependent on PI3K/AKT signaling. Conclusion Overall, these results reveal a novel regulatory mechanism and shed light on how breast tumor cells acquire Postn expression. This complex regulation is likely to be cell type and cancer specific as well as have important therapeutic implications.

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