期刊
ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE
卷 11, 期 10, 页码 450-459出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/2221-1691.326099
关键词
-
资金
- Ministry of Oceans and Fisheries, Republic of Korea [20150242]
The ethanol extract of Chondracanthus tenellus showed potential anti-inflammatory and antioxidant effects by downregulating NF-kappa B, MAPKs, and PI3K/Akt signaling pathways in LPS-stimulated RAW 264.7 macrophages.
Objective: To investigate whether the ethanol extract of Chondracanthus tenellus (Harvey) Hommersand, a type of red algae, could exhibit anti-inflammatory potential in lipopolysaccharide (LPS)-stimulated macrophages.Methods: The ethanol extract of Chondracanthus tenellus was applied to 100 ng/mL LPS-stimulated RAW 264.7 cells, and cell viability, phagocytic ability, levels of pro-inflammatory factors, and the production of reactive oxygen species were measured. To identify the underlying mechanism of the ethanol extract of Chondracanthus tenellus, the expression of inflammation-regulated genes was estimated.Results: The ethanol extract of Chondracanthus tenellus had no cytotoxic effect at concentrations below 300 mu g/mL, and reduced the LPS-induced production of inflammatory mediators including nitric oxide (NO) and prostaglandin E2. Furthermore, the extract markedly suppressed the expression of inducible NO synthase and cyclooxygenase-2, as well as the production of reactive oxygen species. The LPS-induced up-regulation of pro-inflammatory cytokines was attenuated by treatment with the ethanol extract of Chondracanthus tenellus, reducing their extracellular secretion. The Chondracanthus tenellus extract also inhibited LPS-mediated activation of nuclear factor-kappa B (NF-kappa B). In addition, the phosphorylation of mitogen activated protein kinases (MAPKs) and phosphatidylinositol 3 kinase (PI3K)/Akt was markedly increased by LPS, which was significantly abolished by the Chondracanthus tenellus extract.Conclusions: Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-kappa B, MAPKs, and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据