Soil microbial trait-based strategies drive metabolic efficiency along an altitude gradient

Trait-based approaches provide a candidate framework for linking soil microbial community to ecosystem processes, yet how the trade-offs in different microbial traits regulate the community-level metabolic efficiency remains unknown. Herein we assessed the roles of the microbial taxa with particular trait strategies in mediating soil microbial metabolic efficiency along an altitude gradient on the Tibetan Plateau. Results showed that soil microbial metabolic efficiency declined with increasing altitude, as indicated by the increasing metabolic quotient (microbial respiration per unit biomass, qCO(2)) and decreasing carbon use efficiency (CUE). Both qCO(2) and CUE were predominantly predicted by microbial physiological and taxonomic attributes after considering key environmental factors including soil pH, substrate quantity and quality. Specifically, the reduced metabolic efficiency was associated with higher investment into nutrient (particularly for phosphorus) acquisitions via enzymes. Furthermore, we identified key microbial assemblies selected by harsh environments (low substrate quality and temperature) as important predictors of metabolic efficiency. These results suggest that particular microbial assemblies adapted to nutrient limited and cold habitats, but at the expense of lower metabolic efficient at higher altitude. Our findings provide a candidate mechanism underlying community-level metabolic efficiency, which has important implications for microbial-mediated processes such as carbon dynamics under global climate changes.

Automated summary

Trait-based approaches provide a framework for linking soil microbial community to ecosystem processes, but the trade-offs in different microbial traits regulating metabolic efficiency at community level remains unknown. In Tibetan Plateau, soil microbial metabolic efficiency declined with altitude increase, influenced by microbial physiological and taxonomic attributes. Specific microbial assemblies investing in nutrient acquisition, particularly phosphorus, were associated with lower metabolic efficiency at higher altitudes.