Enhanced antibacterial activity of acid treated MgO nanoparticles on Escherichia coli

Acid treatment is one of the effective methods that directly modifies surface physical and chemical properties of inorganic materials, which improves the materials' application potential. In this work, the surface modified MgO nanoparticles (NPs) were prepared through a facile acid-treatment method at room temperature. Compared with the untreated sample, the surviving Escherichia coli (E. coli, ATCC 25922) colonies of the modified MgO NPs decreased from 120 to 54 (10(2) CFU mL(-1)). The enhanced antibacterial activity may be due to the improvement of oxygen vacancies and absorbed oxygen (O-A) content (from 41.6% to 63.1%) as confirmed by electron spin resonance (ESR) and X-ray photoelectron spectroscopy (XPS). These findings revealed that the acid treatment method could directly modify the surface of MgO NPs to expose more oxygen vacancies, which would promote reactive oxygen species (ROS) generation. The membrane tube and single ROS scavenging results further indicated that the increased antibacterial ability originated from the synergetic effect of ROS damage (especially O-2(-)) and direct contact between H-MgO NPs and E. coli.

Automated summary

In this study, surface modified MgO nanoparticles were prepared through acid treatment, resulting in enhanced antibacterial activity, possibly due to increased oxygen vacancies and absorbed oxygen content. The acid treatment method can directly modify the surface of MgO nanoparticles to expose more oxygen vacancies, promoting the generation of reactive oxygen species and enhancing antibacterial ability.