期刊
CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH
卷 24, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.coesh.2021.100308
关键词
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资金
- Catalan Government [2020PANDE00044]
- CDTI project PROCEED [EXP -00132149/COI-20201289]
Researchers summarized the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discussed their advantages and disadvantages, while providing preliminary results of two sensitive and cost-effective research methods. Next-generation sequencing approaches allow for simultaneous detection of signature mutations of different variants of concern, whereas targeted PCR methods require continuous updating but are sensitive and cost-effective.
SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective.
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