期刊
JOURNAL OF OLEO SCIENCE
卷 70, 期 4, 页码 581-587出版社
JAPAN OIL CHEMISTS SOC
DOI: 10.5650/jos.ess20331
关键词
biodegradation; biosurfactant; cyclic lipopeptide; Pseudomonas putida; surfactin
资金
- Kaneka Corporation, Japan
By studying the microbial communities in two enrichment cultures, it was found that various bacterial species dominated the degradation of surfactin. One strain, Pseudomonas putida, was identified as a bacterium with strong surfactin-degrading ability.
A total of 100 environmental samples were investigated for their ability to degrade 1 g/L surfactin as a substrate. Among them, two enrichment cultures, which exhibited microbial growth as well as surfactin degradation, were selected and further investigated. After several successive cultivations, nanopore sequencing of full-length 16S rRNA genes with MinION (TM) was used to analyze the bacterial species in the enrichment cultures. Variovorax spp., Caulobacter spp., Sphingopyxis spp., and Pseudomonas spp. were found to be dominant in these surfactin-degrading mixed cultures. Finally, one strain of Pseudomonas putida was isolated as a surfactin-degrading bacterium. This strain degraded 1 g/L surfactin below a detectable level within 14 days, and C-13 surfactin was degraded faster than C-15 surfactin.
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