期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 97, 期 -, 页码 90-96出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2016.11.008
关键词
Laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS); Parylene-matrix chip; Antibiotic susceptibility test; beta-lactamase
资金
- National Research Foundation of Korea [2014M3A9E5073818]
- Korean Small and Medium Business Administration [S2220656]
- Korea Evaluation Institute of Industrial Technology (KEIT) [S2220656] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2014M3A9E5073818] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Many kinds of susceptibility test for P-lactam antibiotics have been used to determine the antibiotic resistance of bacterial strains. Here, a sensitive antibiotic susceptibility test was presented by using a specialized reaction tool for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) based on parylene-matrix chip. The p-lactamase assay was carried out in a specialized reaction tool by (1) concentrating the bacterial strain and (2) incubating the bacteria with penicillin-G. The parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array, and the products of p-lactamase reaction in the low range of mass-to-charge ratio (m/z< 500) could be effectively analyzed by using a parylene-matrix chip. The sensing parameters were compared with conventional chromogenic antibiotic susceptibility test for beta-lactam antibiotics. Finally, LDI-TOF MS with a specialized reaction tool and parylene-matrix chip could achieve a limit of detection as low as 600 cells/spot for penicillin-G. (C) 2016 Elsevier Inc. All rights reserved.
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