期刊
G3-GENES GENOMES GENETICS
卷 11, 期 12, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkab338
关键词
eyeless; chromatin domain boundary; polycomb response elements; matrix-associated regions; long-range interaction; chromatin domain; gene regulation; Drosophila
资金
- Council of Scientific and Industrial Research (CSIR), India
The study revealed the regulatory mechanisms maintaining the unique expression pattern of the eyeless gene in Drosophila, involving boundary elements ME and EB, as well as a newly identified element ey-PRE. These findings suggest a general regulatory mechanism for the maintenance of genes in functionally independent chromatin domains.
In Drosophila, expression of eyeless (ey) gene is restricted to the developing eyes and central nervous system. However, the flanking genes, myoglianin (myo), and bent (bt) have different temporal and spatial expression patterns as compared to the ey. How distinct regulation of ey is maintained is mostly unknown. Earlier, we have identified a boundary element intervening myo and ey genes (ME boundary) that prevents the crosstalk between the cis-regulatory elements of myo and ey genes. In the present study, we further searched for the cis-elements that define the domain of ey and maintain its expression pattern. We identify another boundary element between ey and bt, the EB boundary. The EB boundary separates the regulatory landscapes of ey and bt genes. The two boundaries, ME and EB, show a long-range interaction as well as interact with the nuclear architecture. This suggests functional autonomy of the ey locus and its insulation from differentially regulated flanking regions. We also identify a new Polycomb Response Element, the ey-PRE, within the ey domain. The expression state of the ey gene, once established during early development is likely to be maintained with the help of ey-PRE. Our study proposes a general regulatory mechanism by which a gene can be maintained in a functionally independent chromatin domain in gene-rich euchromatin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据