期刊
EMERGING MICROBES & INFECTIONS
卷 10, 期 1, 页码 2279-2290出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2021.2008772
关键词
Yellow fever 17D; mouse models; correlate of protection; type I IFN response; live-attenuated vaccine
资金
- European Union's Horizon 2020 research and innovation program [733176]
- Research Foundation Flanders (FWO) under the Excellence of Science (EOS) program [30981113]
- Belgian Science Policy Office (BELSPO) programme Interuniversitaire attractiepolen (IUAP)
- KU Leuven Rega Foundation
- KU Leuven Internal Funds [C3/19/057]
- Chinese Scholarship Council (CSC) [201706760059]
- H2020 Societal Challenges Programme [733176] Funding Source: H2020 Societal Challenges Programme
Research evaluated the immunogenicity and protective efficacy of the live-attenuated yellow fever 17D (YF17D) vaccine in various mouse models, highlighting the model-dependent differences in vaccine-induced protective immunity.
The live-attenuated yellow fever 17D (YF17D) vaccine is one of the most efficacious human vaccines and also employed as a vector for novel vaccines. However, in the lack of appropriate immunocompetent small animal models, mechanistic insight in YF17D-induced protective immunity remains limited. To better understand YF17D vaccination and to identify a suitable mouse model, we evaluated the immunogenicity and protective efficacy of YF17D in five complementary mouse models, i.e. wild-type (WT) BALB/c, C57BL/6, IFN-alpha/beta receptor (IFNAR(-/-) ) deficient mice, and in WT mice in which type I IFN signalling was temporally ablated by an IFNAR blocking (MAR-1) antibody. Alike in IFNAR(-/-) mice, YF17D induced in either WT mice strong humoral immune responses dominated by IgG2a/c isotype (Th1 type) antibodies, yet only when IFNAR was blocked. Vigorous cellular immunity characterized by CD4(+) T-cells producing IFN-gamma and TNF-alpha were mounted in MAR-1 treated C57BL/6 and in IFNAR(-/-) mice. Surprisingly, vaccine-induced protection was largely mouse model dependent. Full protection against lethal intracranial challenge and a massive reduction of virus loads was conferred already by a minimal dose of 2 PFU YF17D in BALB/c and IFNAR(-/-) mice, but not in C57BL/6 mice. Correlation analysis of infection outcome with pre-challenge immunological markers indicates that YFV-specific IgG might suffice for protection, even in the absence of detectable levels of neutralizing antibodies. Finally, we propose that, in addition to IFNAR(-/-) mice, C57BL/6 mice with temporally blocked IFN-alpha/beta receptors represent a promising immunocompetent mouse model for the study of YF17D-induced immunity and evaluation of YF17D-derived vaccines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据