期刊
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
卷 49, 期 1, 页码 699-708出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2021.2013250
关键词
Spinal cord injury; silk fibroin; nanofibrous structure; porous scaffold; mesenchymal stem cells
资金
- Natural Science Foundation of Jiangsu Province [BK20200208, BK20191168]
- Huai'an health research project [HAWJ202012]
- Suzhou Planning Project of Science and Technology [SYS2019005, SYS2020064]
The study demonstrated the potential therapeutic effects of a three-dimensional porous silk fibroin scaffold seeded with bone marrow stromal cells in a spinal cord injury model, leading to improved regeneration at the injury site and enhanced hindlimb movement recovery as shown by increased BBB scores.
Spinal cord injury (SCI) is a common pathology often resulting in permanent loss of sensory, motor, and autonomic function. Numerous studies in which stem cells have been transplanted in biomaterial scaffolds into animals have demonstrated their considerable potential for recovery from SCI. In the present study, a three-dimensional porous silk fibroin (SF) scaffold with a mean pore size of approximately 383 mu m and nanofibrous structure was fabricated, the silk scaffold enabling the enhanced attachment and proliferation of bone marrow stromal cells (BMSCs). Investigation of its therapeutic potential was conducted by implantation of the nanofibrous SF scaffold seeded with BMSCs into a transected spinal cord model. Recovery of the damaged spinal cord was significantly improved after 2 months, compared with a non-nanofibrous scaffold, in combination with decreased glial fibrillary acidic protein (GFAP) expression and improved axonal regeneration at the site of injury. Furthermore, elevated Basso-Beattie-Bresnahan (BBB) scores indicated greatly improved hindlimb movement. Together, these results demonstrate that transplantation of neural scaffolds consisting of nanofibrous SF and BMSCs is an attractive strategy for the promotion of functional recovery following SCI.
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