4.1 Article

Protocol for cortical-wide field-of-view two-photon imaging with quick neonatal adeno-associated virus injection

期刊

STAR PROTOCOLS
卷 2, 期 4, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.xpro.2021.101007

关键词

-

资金

  1. Japan So-ciety for the Promotion of Science (JSPS) KAKENHI [JP19J01973, JP20H05775]
  2. AMED-Brain/Minds Project [21dm0207001h0008]

向作者/读者索取更多资源

This AAV induction approach enables efficient neuronal labeling with Ca2+ sensors and allows for calcium imaging experiments throughout the entire cortical area in mice.
We recently established a simple and versatile adeno-associated virus (AAV) induction approach that enables dense (>90% labeled neurons) and cortical-wide Ca2+ sensor expression. Here, we describe the stepwise protocol for neonatal AAV injection of a Ca2+ sensor. We also detail the steps for subsequent craniotomy to generate a chronic cranial window, followed by wide-field two-photon Ca2+ imaging in an awake mouse. This protocol serves as an alternative to the use of transgenic animals and offers translatable options for cortical-wide experiments.For complete details on the use and execution of this protocol, please refer to Ota et al. (2021).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据