Combining low-density cell culture, single-cell tracking and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs main-tain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cere-bellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch -clamp recordings. For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).

Automated summary

Culturing postnatal cerebellar neural stem cells (NSCs) in low-density cell culture combined with live imaging and single-cell tracking allows monitoring of their behavior and differentiation into neuronal progeny. The protocol maintains the neurogenic nature of NSCs, generating neuronal progeny in proportions that mimic in vivo conditions, and identifies the progeny through post-imaging immunocytochemistry and patch-clamp recordings referenced in Paniagua-Herranz et al. (2020b).