期刊
STAR PROTOCOLS
卷 2, 期 4, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100988
关键词
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资金
- German Research Foundation (DFG) : Cluster of Excellence REBIRTH [ZW 64/4-2, KFO311/ZW64/7-1, 01EK1602A]
- German Ministry for Education and Science (BMBF) [13N14086, 01EK1601A, 655 13XP5092B, 031L0249, ZN3340]
- Forder-ung aus Mitteln des Niedersaechsischen Vorab [EXC 62/3]
- [ZW64/4-1]
The protocol outlines controlled expansion of hPSCs in stirred tank bioreactors, achieving high cell densities while reducing culture medium consumption. It provides a detailed blueprint for systematic process development of hPSCs and their progenies.
The routine therapeutic and industrial applications of human pluripotent stem cells (hPSCs) require their constant mass supply by robust, efficient, and economically viable bioprocesses. Our protocol describes the fully controlled expansion of hPSCs in stirred tank bioreactors (STBRs) enabling cell densities of 35 3 106 cells/mL while reducing culture medium consumption by 75%. This is achieved by in silico process modeling and computable upscaling. We provide a detailed blueprint for systematic process development of hPSCs and their progenies.For complete details on the use and execution of this protocol, please refer to Manstein et al. (2021).
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