Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells

This protocol is intended as a guide for implementing or refining the usage of the air-liquid interface (ALI) model system to generate airway mucociliary tissue in vi-tro. We present a streamlined protocol for isolating the stem cells from inferior nasal turbinates of donors, allowing for a simple and low-cost supply of primary cells for research. We also provide our detailed protocols for ALI tissue process-ing and immunofluorescence to aid in the standardization of these techniques between research groups. For complete details on the use and execution of this protocol, please refer to Hussain et al., (2014) Yang et al., (2016) Im et al., (2019).

Automated summary

This article provides a guide for generating airway mucociliary tissue in vitro, including protocols for isolating stem cells, ALI tissue processing, and immunofluorescence, to aid in standardizing techniques across research groups. It refers to complete details on the protocol's use and execution in previous studies by Hussain et al. (2014), Yang et al. (2016), and Im et al. (2019).