Optimized workflow for human PBMC multiomic immunosurveillance studies

Summary: Single-cell measurements play a key role in understanding diverse pathways of differentiation, cellular responses to signals, and human diseases. Through innovative technologies, a novel assay has been developed that allows simultaneous measurement of cell surface markers and chromatin accessibility, providing a new toolkit to identify type-specific gene regulation and expression.

Summary: This study demonstrates minimal effects of delayed processing on the numbers and general phenotype of PBMC, but significant changes in the single-cell transcriptome and composition of the plasma proteome can be observed as early as 6 hours after blood draw. These changes reflect patterns of cellular activation across diverse cell types, potentially confounding relevant biologic variance related to disease states.