期刊
BIOENGINEERING-BASEL
卷 8, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/bioengineering8020019
关键词
lymph node-on-a-chip; hydroxychloroquine; motility; rotational motion; reactive oxygen species
资金
- Department of Health [RC2-2018-022]
- Department of Economic Development in Abu Dhabi, UAE [RC2-2018-022]
- Khalifa University of Science and Technology [RC2-2018-022]
Organs On-a-Chip are novel platforms for modeling human physiology and disease, with LN-on-a-chip being a useful tool for drug screening and cellular dynamics studies. Using this innovative technology, researchers were able to monitor real-time motility and viability of B and T cells exposed to HCQ, and observed changes in cell dynamics induced by the drug.
Organs On-a-Chip represent novel platforms for modelling human physiology and disease. The lymph node (LN) is a relevant immune organ in which B and T lymphocytes are spatially organized in a complex architecture, and it is the place where the immune response initiates. The present study addresses the utility of a recently designed LN-on-a-chip to dissect and understand the effect of drugs delivered to cells in a fluidic multicellular 3D setting that mimics the human LN. To do so, we analyzed the motility and viability of human B and T cells exposed to hydroxychloroquine (HCQ). We show that the innovative LN platform, which operates at a microscale level, allows real-time monitoring of co-cultured B and T cells by imaging, and supports cellular random movement. HCQ delivered to cells through a constant and continuous flow induces a reduction in T cell velocity while promotes persistent rotational motion. We also find that HCQ increases the production of reactive oxygen species in T cells. Taken together, these results highlight the potential of the LN-on-a-chip to be applied in drug screening and development, and in cellular dynamics studies.
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