期刊
CYBORG AND BIONIC SYSTEMS
卷 2021, 期 -, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.34133/2021/9864212
关键词
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资金
- NSF [1927628, 1949909]
- NIH SC1 grant [RL5GM118969]
- National Institute of General Medical Sciences of the National Institutes of Health [TL4GM118971, 8UL1GM118970-02]
- NIH BUILD Pilot grant [1828268]
- [1SC1HL154511-01]
In this study, a high-throughput scalable 3D bioprinted cardiac spheroidal droplet-organoid model was designed and developed, offering potential applications in drug screening and regenerative engineering. The model demonstrated robust biocompatibility, structural stability, and supported long-term growth and proliferation of cardiac cells.
Since conventional human cardiac two-dimensional (2D) cell culture and multilayered three-dimensional (3D) models fail in recapitulating cellular complexity and possess inferior translational capacity, we designed and developed a high-throughput scalable 3D bioprinted cardiac spheroidal droplet-organoid model with cardiomyocytes and cardiac fibroblasts that can be used for drug screening or regenerative engineering applications. This study helped establish the parameters for bioprinting and cross-linking a gelatin-alginate-based bioink into 3D spheroidal droplets. A flattened disk-like structure developed in prior studies from our laboratory was used as a control. The microstructural and mechanical stability of the 3D spheroidal droplets was assessed and was found to be ideal for a cardiac scaffold. Adult human cardiac fibroblasts and AC16 cardiomyocytes were mixed in the bioink and bioprinted. Live-dead assay and flow cytometry analysis revealed robust biocompatibility of the 3D spheroidal droplets that supported the growth and proliferation of the cardiac cells in the long-term cultures. Moreover, the heterocellular gap junctional coupling between the cardiomyocytes and cardiac fibroblasts further validated the 3D cardiac spheroidal droplet model.
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