期刊
FEMS MICROBIOLOGY ECOLOGY
卷 97, 期 11, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiab145
关键词
denitrification; autotrophic; NRFeOx; aquifer; metagenomics; Gallionellaceae
类别
资金
- Emmy Noether fellowship (DFG) [326028733]
- Institutional Strategy of the University of Tubingen (German Research Foundation
- Deutsche Forschungsgemeinschaft [DFG]) [Zukunftskonzept [ZUK] 63]
- Collaborative Research Center 1253 CAMPOS (Project 5: Fractured Aquifers - German Research Foundation (DFG) [1253/1 2017]
The presence of Fe(II) promotes the growth of denitrifying Burfeholderiaceae spp. and an unclassified Gallionellaceae sp., with the latter showing the potential to oxidize Fe(II), fix CO2, and perform denitrification. Novel putative NRFeOx taxa, including Curvibacter spp., Methyloversatilis sp., and Thermomonas spp., were also identified.
Autotrophic nitrate reduction coupled to Fe(II) oxidation is an important nitrate removal process in anoxic aquifers. However, it remains unknown how changes of O-2 and carbon availability influence the community structure of nitrate-reducing Fe(II)-oxidizing (NRFeOx) microbial assemblages and what the genomic traits of these NRFeOx key players are. We compared three metabolically distinct denitrifying assemblages, supplemented with acetate, acetate/Fe(II) or Fe(II), enriched from an organic-poor, pyrite-rich aquifer. The presence of Fe(II) promoted the growth of denitrifying Burfeholderiaceae spp. and an unclassified Gallionellaceae sp. This Gallionellaceae sp. was related to microaerophilic Fe(II) oxidizers; however, it did not grow under microoxic conditions. Furthermore, we explored a metagenome and 15 metagenome-assembled genomes from an aquifer-originating, autotrophic NRFeOx culture. The dominant Gallionellaceae sp. revealed the potential to oxidize Fe(II) (e.g. cyc2), fix CO2 (e.g. rbcL) and perform near-complete denitrification leading to N2O formation (e.g. narGHJI, nirK/S and norBC). In addition, Curvibacter spp., Methyloversatilis sp. and Thermomonas spp. were identified as novel putative NRFeOx taxa. Our findings provide first insights into the genetic traits of the so far only known autotrophic NRFeOx culture originating from an organic-poor aquifer, providing the genomic basis to study mechanisms of nitrate removal in organic-poor subsurface ecosystems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据