期刊
CURRENT ISSUES IN MOLECULAR BIOLOGY
卷 43, 期 3, 页码 2276-2288出版社
MDPI
DOI: 10.3390/cimb43030160
关键词
Cyprinus carpio haematopterus; modified GBS method; linkage map; dynamic QTL; growth-related trait
资金
- National Natural Science Foundation of China [31602149]
- Science and Technology Project of Henan Province [192102110083]
- Starting Fund for Doctoral Research of Henan Normal University [QD18095]
This study constructed three genetic linkage maps of the Yellow River carp and identified 148 QTLs associated with four growth traits. The results suggest the presence of growth-related genes on chromosome 12, with 13 main QTLs located on this chromosome. Detected candidate genes within the vicinity of the main QTLs provide potential targets for further molecular-assisted breeding research.
To provide the theoretical basis for researching growth, development, and molecular marker-assisted breeding of the economically important Yellow River carp (Cyprinus carpio haematopterus) using dynamic quantitative trait locus (QTL) mapping, we constructed three genetic linkage maps from 207 progeny using a new modified genotyping-by-sequencing method. The three maps contained 16,886, 16,548, and 7482 single nucleotide polymorphism markers, respectively, with an average interval of 0.36 cM, 0.45 cM, and 1.00 cM. We identified 148 QTLs related to four growth traits that were located on 25 chromosomes from three growth stages of Yellow River carp. A total of 32, 36, 43, and 37 QTLs were associated with body length, height, width, and weight, respectively. Among them, 47 QTLs were detected for only one growth trait in one stage, but all of the other QTLs were co-localized. Of the 14 main QTLs, 13 were located on chromosome 12, which suggests the presence of growth-related genes on this chromosome. We then detected 17 candidate genes within 50 K upstream and downstream of the 14 main QTLs. This is the first report of the dynamic QTL mapping of growth traits of Yellow River carp, and the results can be used in future studies of growth, development, and molecular-assisted breeding of this species.
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