期刊
FUNCTION
卷 2, 期 1, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/function/zqaa033
关键词
satellite cell; muscle stem cell; Pax7; exercise; hypertrophy; adaptation; muscle function
资金
- National Institutes of Health (NIH) [AR060701, AG049806, AR075364, AR071753, AG063994, HL121284, HL136951, AR073638]
- NIH National Center for Advancing Translational Sciences [TL1TR001997]
Satellite cells play a crucial role in skeletal muscle hypertrophy, as shown by a study on mice subjected to progressive weighted wheel running. While muscle can still exhibit hypertrophic response without satellite cells, growth and adaptation are ultimately impaired with altered gene networks.
Satellite cells are required for postnatal development, skeletal muscle regeneration across the lifespan, and skeletal muscle hypertrophy prior to maturity. Our group has aimed to address whether satellite cells are required for hypertrophic growth in mature skeletal muscle. Here, we generated a comprehensive characterization and transcriptome-wide profiling of skeletal muscle during adaptation to exercise in the presence or absence of satellite cells in order to identify distinct phenotypes and gene networks influenced by satellite cell content. We administered vehicle or tamoxifen to adult Pax7-DTA mice and subjected them to progressive weighted wheel running (PoWeR). We then performed immunohistochemical analysis and whole-muscle RNA-seq of vehicle (SC+) and tamoxifen-treated (SC- mice. Further, we performed single myonuclear RNA-seq to provide detailed information on how satellite cell fusion affects myonuclear transcription. We show that while skeletal muscle can mount a robust hypertrophic response to PoWeR in the absence of satellite cells, growth, and adaptation are ultimately blunted. Transcriptional profiling reveals several gene networks key to muscle adaptation are altered in the absence of satellite cells. [GRAPHICS]
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