期刊
ENVIRONMENTAL MICROBIOLOGY
卷 19, 期 3, 页码 1041-1053出版社
WILEY
DOI: 10.1111/1462-2920.13605
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资金
- U. S. Department of Energy (DOE), Office of Science, Office of Biological and Environmental Research
- ORNL Plant-Microbe Interfaces Scientific Focus Area project
- DOE [DOE-SC10010566]
- NSF [OCE-1233964]
- United States Department of Energy [DE-AC05-00OR22725]
- Direct For Biological Sciences
- Division Of Environmental Biology [1441715] Funding Source: National Science Foundation
Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant-fungal-endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations and allosteric effectors. Such multimodal regulations may be a general mechanism for metabolic modulation.
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