期刊
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
卷 35, 期 1, 页码 1858-1868出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13102818.2021.2023327
关键词
Bupleurum chinense DC; coumarins; key genes; transcriptome; metabolome
资金
- China Agriculture Research System of MOF
- China Agriculture Research System of MARA
- Young Elite Scientists Sponsorship Program by CACM [2019-QNRC2-C15]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2M-2-003]
- Crop and livestock Breeding Project in Sichuan [2021YFYZ0012]
- Programs of Science and Technology Department of Sichuan Province [2019YFH0072]
- Doctoral Research Funding of Southwest University of Science and Technology [19zx7117]
- China's Ministry of Foreign Affairs 2017 Asia Regional Cooperation Special Fund Project [[2016]318]
- Programs of Sichuan Provincial Administration of Traditional Chinese Medicine [A-2017N-18]
By analyzing transcriptome and metabolomic data, this study identified 89 differentially expressed genes involved in coumarin biosynthesis pathways in Bupleurum chinense. Correlation network analysis pinpointed key genes affecting coumarin biosynthesis, such as psoralen synthase, caffeic acid omicron-methyltransferase, and shikimate O-hydroxycinnamoyltransferase.
Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in B. chinense, using transcriptome and metabolomic data obtained by high-throughput RNA-sequencing data analysis, and liquid chromatography tandem mass spectrometry. Five coumarin compounds: esculetin (2), fraxetin (4), psoralen (5), parmesan (6) and angelicin (7) were identified through metabolomic analysis. A total of 39 unigenes belonging to five gene families: shikimate O-hydroxycinnamoyltransferase (HCT), caffeic acid omicron-methyltransferase (COMT), 4-coumarate: coenzyme A ligase (4CL), feruloyl-CoA 6'-hydroxylase (F6'H) and psoralen synthase (PS), were identified by correlating coumarin content with transcriptional changes. According to correlation network analysis, PS genes (Bc09210, Bc12711), COMT genes (Bc14830, Bc17539, Bc28792, Bc32856) and HCT genes (Bc32814, Bc10245) may be important factors affecting coumarin biosynthesis.
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