Multi-year progesterone profiles during pregnancy in baleen of humpback whales (Megaptera novaeangliae)

Understanding calving rates of wild whale populations is critically important formanagementand conservation. Reproduction of humpback whales (Megaptera novaeangliae) is difficult to monitor and, even with long-term sighting studies, basic physiological information such as pregnancy rates and calving intervals remain poorly understood in many populations. We hypothesized that pregnant whales have sustained elevations in baleen progesterone that temporally correlate with gestation. To test this hypothesis, baleen progesterone profiles from two adult female North Pacific humpbacks, both with extensive sighting records and documented pregnancies, were compared to those of a nulliparous female (adult female never seen with a calf) and a juvenile male. Baleen specimens recovered during necropsywere subsampled every 2 cmfromthe base to the tip of the plate, with each interval representing 30-45 days of growth. Homogenized baleen powder was assayed for progesterone using enzyme immunoassays. The date of growth of each sampling location on the baleen plate was estimated based on stable isotope analysis of annual delta N-15 cycles. Progesterone profiles from both pregnant whales showed sustained high progesterone content (>350 ng/g) in areas corresponding to known pregnancies, inferred from calf sightings and post-mortemdata. The younger female, estimated to be 13 years old, had higher progesterone during pregnancy than the 44.5 year old, but levels during non-pregnancy were similar. The nulliparous female and the male had low progesterone throughout their baleen plates. Baleen hormone analysis can determine how progesterone concentrations change throughout gestation and has potential for estimating age at first reproduction, pregnancy intervals, failed pregnancies and early calf mortality. Understanding rates of calving and current and historic reproductivepatterns in humpbacks is vital to continuing conservation measures in this species.

Automated summary

Understanding calving rates of wild whale populations is crucial for management and conservation efforts. This study used baleen hormone analysis to investigate progesterone levels in pregnant humpback whales, with implications for estimating reproductive parameters such as age at first reproduction, pregnancy intervals, and early calf mortality.