Fast Cardiac Imaging in Live Embryos Using Multiphoton Light-sheet Microscopy at Low Laser Repetition Rate

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

Automated summary

This study presents a framework for quantifying photodamage in multiphoton light-sheet microscopy, and demonstrates a significant signal enhancement by adjusting the laser repetition rate during cardiac imaging in live zebrafish embryos.