Enhanced Antibiofilm Activity of Endophytic Bacteria Mediated Zirconium Nanoparticles

Unraveling the biofilm formation of oral pathogens by endophytic bacteria-mediated zirconia nanoparticles promotes to exhibits as a dental restorative agent by enhancing antibiofilm activity. To demonstrate the mechanistic studies of biogenic zirconium nanoparticle restricts the growth of oral pathogens. The endophytic bacteria isolated from Terminalia chebula have valuable secondary metabolites used as reducing agents for the synthesis of zirconia nanoparticles. Characterization studies were done for the application of dental restorative material. The nanospheres' shape and size were confirmed by SEM/EDAX followed by XRD and FTIR for their chemical groups that contribute as the antagonist for biofilm. Parallelly evaluation of antioxidant, antibacterial, anti-inflammatory, and antibiofilm activity is opposing the disease-causing pathogens and examined for biocompatibility on Human Primary Gingival fibroblast cell lines. Therefore, the endophytic bacteria mediated zirconia nanoparticles were biologically assessed against oral pathogens, promoting results to exhibit dental restorative biomaterial.

Automated summary

The biofilm formation of oral pathogens was studied using endophytic bacteria-mediated zirconia nanoparticles, which enhanced the antibiofilm activity and displayed potential as a dental restorative agent. Biogenic zirconium nanoparticles synthesized using reducing agents from Terminalia chebula, an endophytic bacteria, were characterized for their dental restorative application. The shape and size of nanospheres were confirmed by SEM/EDAX, and their chemical groups were analyzed using XRD and FTIR, which showed antagonistic effects on biofilm. The nanoparticles were evaluated for antioxidant, antibacterial, anti-inflammatory, and antibiofilm activities against disease-causing pathogens and tested for biocompatibility with Human Primary Gingival fibroblast cell lines. Thus, endophytic bacteria-mediated zirconia nanoparticles were biologically assessed against oral pathogens and demonstrated their potential as dental restorative biomaterial.