期刊
ACTA PHARMACEUTICA
卷 72, 期 1, 页码 109-122出版社
HRVATSKO FARMACEUTSKO DRUSTOV (HFD)-CROATION PHARMACEUTICAL SOC
DOI: 10.2478/acph-2022-0005
关键词
Annexin A1; siRNA transfection; apoptosis; cell cycle; phagocytosis; leukemia
资金
- Ministry of Higher Education [FRGS/1/2015/SKK09/UKM/02/3]
Annexin A1 (ANXA1), an endogenous protein, plays important roles in the regulation of proliferation, cell cycle, phagocytosis, and apoptosis in leukemia cells. Knockdown of ANXA1 led to decreased expression and induced apoptosis in leukemia cells. It also affected the expression of caspases and Bcl-2, and enhanced phagocytosis activity. These findings suggest that ANXA1 knockdown might be a potential therapeutic strategy for leukemia.
Annexin A1 (ANXA1) is an endogenous protein involved in the control of proliferation, cell cycle, phagocytosis, and apoptosis in several types of cancer. To investigate the effects of ANXA1 knockdown in leukemia cells, transfection with specific ANXA1 siRNA was performed. Cell cycle and apoptosis were analyzed using flow cytometry and a mecha-nism involving caspases and Bcl-2 was quantified using Western blotting. Phagocytosis activity was evaluated using hematoxylin & eosin staining. The ANXA1 expression was significantly downregulated after the knockdown and apoptosis was induced in tested cells. The expression of caspase-9 and-3 increased in U937 and Jurkat cells respec-tively. Bcl-2 expression was downregulated in K562 and Jurkat cells while upregulated in U937. The number of leu-kemic cells arrested at the G2/M phase and the phagocytosis index were significantly increased in transfected cells. This suggests that ANXA1 knockdown might be a potential approach in the therapeutic strategy for leukemia.
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