期刊
STEM CELL REVIEWS AND REPORTS
卷 18, 期 1, 页码 360-377出版社
SPRINGER
DOI: 10.1007/s12015-021-10266-z
关键词
AggreWell; Co-culture; Fetal mesenchyme; Niche-specific; Pancreas; Spheroid
资金
- Royan Institute for Stem Cell Biology and Technology (RI-SCBT)
- Lotus Charity Investment Foundation, Royan Institute
In this study, the impact of co-culturing human embryonic stem cell-derived pancreatic progenitors with human fetal pancreatic-derived mesenchymal cells on endocrine and beta cell development was evaluated. It was found that pancreatic mesenchyme had an inductive effect on pancreatic progenitors, promoting beta cell maturation. Additionally, scalable cultures combining these cells were investigated for their potential applications in mimicking pancreatic tissue for developmental studies.
Mesenchymal cells of diverse origins differ in gene and protein expression besides producing varying effects on their organ-matched epithelial cells' maintenance and differentiation capacity. Co-culture with rodent's tissue-specific pancreatic mesenchyme accelerates proliferation, self-renewal, and differentiation of pancreatic epithelial progenitors. Therefore, in our study, the impact of three-dimensional (3D) co-culture of human fetal pancreatic-derived mesenchymal cells (hFP-MCs) with human embryonic stem cell-derived pancreatic progenitors (hESC-PPs) development towards endocrine and beta cells was assessed. Besides, the ability to maintain scalable cultures combining hFP-MCs and hESC-PPs was investigated. hFP-MCs expressed many markers in common with bone marrow-derived mesenchymal stem cells (BM-MSCs). However, they showed higher expression of DESMIN compared to BM-MSCs. After co-culture of hESC-PPs with hFP-MCs, the pancreatic progenitor (PP) spheroids generated in Matrigel had higher expression of NGN3 and INSULIN than BM-MSCs co-culture group, which shows an inductive impact of pancreatic mesenchyme on hESC-PPs beta-cells maturation. Pancreatic aggregates generated by forced aggregation through scalable AggreWell system showed similar features compared to the spheroids. These aggregates, a combination of hFP-MCs and hESC-PPs, can be applied as an appropriate tool for assessing endocrine-niche interactions and developmental processes by mimicking the pancreatic tissue.
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