期刊
JACS AU
卷 2, 期 2, 页码 357-366出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacsau.1c00387
关键词
DNA nanotechnology; enzymatic cascades; self-assembly; biomolecular scaffolds; DNA origami
资金
- National Science Foundation [1905920]
- U.S. Department of Energy, Office of Basic Energy Sciences [DE-SC0008772]
- U.S. DOE Office of Science Facilities at Brookhaven National Laboratory [DE-SC0012704]
- U.S. Department of Energy (DOE) [DE-SC0008772] Funding Source: U.S. Department of Energy (DOE)
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1905920] Funding Source: National Science Foundation
DNA nanotechnology is used to structure enzymes in a desired format. In this study, a three-dimensional DNA wireframe octahedron is created to investigate the impact of enzyme spacing, arrangement, and location on cascade activity. The experiments reveal the significance of enzyme colocalization on the DNA scaffold over specific enzyme arrangements.
DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works introducing enzyme organizations in 3D lattices. As the ability to define nanoscale structure has grown, it is critical to fully deconstruct the impact of enzyme organization at the single-scaffold level. Here, we present an open, three-dimensional (3D) DNA wireframe octahedron which is used to create a library of spatially arranged organizations of glucose oxidase and horseradish peroxidase. We explore the contribution of enzyme spacing, arrangement, and location on the 3D scaffold to cascade activity. The experiments provide insight into enzyme scaffold design, including the insignificance of scaffold sequence makeup on activity, an increase in activity at small enzyme spacings of <10 nm, and activity changes that arise from discontinuities in scaffold architecture. Most notably, the experiments allow us to determine that enzyme colocalization itself on the DNA scaffold dominates over any specific enzyme arrangement.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据